Wow. I signed up for this forum specifically to ask this very question about my 2i4, and found this thread right at the top.
The buffer size in the ASIO Control Panel keeps jumping back to 1024, but I'm not sure if it does so when the computer is rebooted, or if it has something to do with launching Cubase. When it's set to 1024, Cubase won't play back correctly, there are large gaps in the audio. If I shorten the buffers to 256 in ASIO Control Panel, playback sounds fine.
What is the relationship between the ASIO buffer size(s) in Cubase's studio config section, and the one set in Focusrite's ASIO Control Panel?